Precipitation and Inhibition of Carboxypeptidase by Specific Antisera

Precipitation and inhibition of carboxypeptidase by specific antisera.

Quantitative immunochemical techniques have been employed in the study of enzyme systems as a method of assessing the homogeneity of the enzyme protein. In addition, when the antibody acts as an enzyme inhibitor, useful information can frequently be obtained. Such studies have recently been reviewed by Marrack (1). With crystalline carboxypeptidase as an antigen, specific antibodies were obtain...

Inhibition of Eimeria acervulina Sporozoite Invasion by Rabbit and Chicken Antisera Using ISI Assay

Inhibition of granulocyte erythrophagocytosis by HLA antisera.

The ability of HLA antisera to inhibit granulocyte erythrophagocytosis (EP) of opsonized red blood cells (RBC) was evaluated. Human granulocytes (PMN) were separated from heparinized whole blood by the Ficoll-Isopaque technique and suspended in McCoy's medium. EP occurred when the PMN were incubated with opsonized RBC. For six HLA antibody specificities evaluated, prior incubation of PMN with H...

Glomerulonephritis Produced in Dogs by Specific Antisera

The intravenous injection of rabbit antidog-placenta or antidog-kidney serum produced immediate evidence of glomerulonephritis in dogs. The lesions produced by the two agents were indistinguishable. The glomerulonephritis so induced may terminate in death within 8 days, may progress to a chronic form or may heal. The acute phase of the nephritis was usually associated with severe exudative and ...

The Inhibition of Streptococcal Desoxyribonuclease by Rabbit and Human Antisera

Rabbit antisera against partially purified streptococcal desoxyribonuclease inhibit the action of the enzyme on its substrate. The activity of pancreatic desoxyribonuclease is not affected by these antisera. Similarly antibody against pancreatic nuclease does not inhibit the streptococcal enzyme. Certain patients develop inhibitory antibody to streptococcal desoxyribonuclease following streptoc...